In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge

Објеката

Тип

Рад у часопису

Верзија рада

објављена верзија

Језик

енглески

Креатор

Aleksandar Mijatović, Nevenka Gligorijević, Dušan Ćoćić, Snežana Spasić, Aleksandar Lolić, Sandra Aranđelović, Milan Nikolić, Rada Baošić

Извор

Journal of Inorganic Biochemistry

Издавач

Elsevier BV

Датум издавања

2023

Сажетак

The biological activity of six structurally similar tetradentate Schiff base copper(II) complexes, namely [Cu (ethylenediamine-bis-acetylacetonate)] (CuAA) and five derivatives where two methyl groups are replaced by phenyl, (CuPP), CF3 (CuTT) or by mixed groups CH3/CF3 (CuAT), Ph/CF3 (CuPT), and Ph/CH3 (CuAP) has been investigated. The set of antioxidant assays was performed, and the results were expressed as IC50 and EC50 values. The series of complexes showed interesting bioactivity and were investigated for the determination of antioxidant, antifungal, antimicrobial, and cytotoxic activity. A significant antioxidant behavior was exhibited by complex CuAA, greater than Trolox in the Oxygen Radical Absorbance Capacity (ORAC) assay. Antibacterial assay over Gram-positive and Gram-negative pathogenic bacterial strains and some fungal pathogens were studied. Antiproliferative activity of complexes in two human tumor cell lines, breast adenocarcinoma MCF-7, colon adenocarcinoma LS-174, and normal fibroblast cells-MRC-5, examined the effect on cell cycle progression. The significant cytotoxic potential, comparable to cisplatin cytotoxicity, was determined in human breast cancer cell line-MCF-7 with IC50 values being 17.53–31.40 μM and human colon cancer cell line-LS-174 with IC50 values being 15.22–23.92 μM. All tested compounds showed nearly twice more selectivity toward cancer cell lines than normal cells. The interactions of complexes with human serum albumin (HSA), the most prominent protein in plasma, were investigated using spectroscopic fluorescence techniques. The complexes bind to human serum albumin at multiple sites (n = 0.2–1.9), displaying a moderate binding constant Ka = 4.1–12.4 × 104 M− 1. The molecular docking experiment effectively showed complex binding to HSA and DNA molecular fragments.

том

244

Број

112224

doi

10.1016/j.jinorgbio.2023.112224

issn

0162-0134

Subject

Inorganic Chemistry,Biochemistry

uri

https://reader.elsevier.com/reader/sd/pii/S016201342300106X?token=980C4ED9C58D4D4A3B24A3B4E680859992B46440776C35996EF754F80EF6574B272A2F51F8EB009C32836D47958DD582&originRegion=eu-west-1&originCreation=20230420074804

Шира категорија рада

M20

Ужа категорија рада

М21

Права

Затворени приступ

Лиценца

Creative Commons – Attribution-Share Alike 4.0 International

Формат

.pdf

ORCID

https://orcid.org/0000-0002-5553-9244

Скупови објеката

Александар Мијатовић

Рада Баошић

Radovi istraživača

Aleksandar Mijatović, Nevenka Gligorijević, Dušan Ćoćić, Snežana Spasić, Aleksandar Lolić, Sandra Aranđelović, Milan Nikolić, Rada Baošić. "In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge" in Journal of Inorganic Biochemistry, Elsevier BV (2023). https://doi.org/10.1016/j.jinorgbio.2023.112224

This item was submitted on 19. април 2023. by [anonymous user] using the form “Рад у часопису” on the site “Радови”: http://drug.rgf.bg.ac.rs/s/repo