Претрага
232 items
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Structure, interaction with biomolecules, and cytotoxicity of copper (II) complexes chelating some Schiff base ligands
Aleksandar Mijatović, Angelina Z. Caković, Aleksandar Lolić, Olivera Klisurić, Marko N. Živanović, Dragana S. Šeklić, Snežana Sretenović, Marija Ilić, Jovana Bogojeski (2023)Cancer remains one of the world's deadliest diseases, claiming many lives each day. Transition metal complexes are leading candidates in developing anticancer agens with cisplatin used in chemotherapy worldwide. Copper, an endogenous metal in the human body, is especially attractive because of its typical redox biological activities and a relatively strong affinity to nucleobases. A series of Cu(II) complexes with ethane-1,2-diamine as the amine part and pentane-2,4-dione and/or 1-phenylbutane-1,3-dione, pentane-2,4-dione and/or 1,1,1- trifluoropentane-2,4-dione, or 1,1,1-trifluoropentane-2,4-dione and/or 1-phenylbutane-1,3-dione as the ...Aleksandar Mijatović, Angelina Z. Caković, Aleksandar Lolić, Olivera Klisurić, Marko N. Živanović, Dragana S. Šeklić, Snežana Sretenović, Marija Ilić, Jovana Bogojeski. "Structure, interaction with biomolecules, and cytotoxicity of copper (II) complexes chelating some Schiff base ligands" in Applied Organometallic Chemistry, Wiley (2023). https://doi.org/10.1002/aoc.7253
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In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge
Aleksandar Mijatović, Nevenka Gligorijević, Dušan Ćoćić, Snežana Spasić, Aleksandar Lolić, Sandra Aranđelović, Milan Nikolić, Rada Baošić (2023)The biological activity of six structurally similar tetradentate Schiff base copper(II) complexes, namely [Cu(ethylenediamine-bis-acetylacetonate)] (CuAA) and five derivatives where two methyl groups are replaced by phenyl, (CuPP), CF3 (CuTT) or by mixed groups CH3/CF3 (CuAT), Ph/CF3 (CuPT), and Ph/CH3 (CuAP) has been investigated. The set of antioxidant assays was performed, and the results were expressed as IC50 and EC50 values. The series of complexes showed interesting bioactivity and were investigated for the determination of antioxidant, antifungal, antimicrobial, and cytotoxic ...Aleksandar Mijatović, Nevenka Gligorijević, Dušan Ćoćić, Snežana Spasić, Aleksandar Lolić, Sandra Aranđelović, Milan Nikolić, Rada Baošić. "In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge" in Journal of Inorganic Biochemistry, Elsevier BV (2023). https://doi.org/10.1016/j.jinorgbio.2023.112224
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In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge
Aleksandar Mijatović, Nevenka Gligorijević, Dušan Ćoćić, Snežana Spasić, Aleksandar Lolić, Sandra Aranđelović, Milan Nikolić, Rada Baošić (2023)The biological activity of six structurally similar tetradentate Schiff base copper(II) complexes, namely [Cu (ethylenediamine-bis-acetylacetonate)] (CuAA) and five derivatives where two methyl groups are replaced by phenyl, (CuPP), CF3 (CuTT) or by mixed groups CH3/CF3 (CuAT), Ph/CF3 (CuPT), and Ph/CH3 (CuAP) has been investigated. The set of antioxidant assays was performed, and the results were expressed as IC50 and EC50 values. The series of complexes showed interesting bioactivity and were investigated for the determination of antioxidant, antifungal, antimicrobial, and ...Aleksandar Mijatović, Nevenka Gligorijević, Dušan Ćoćić, Snežana Spasić, Aleksandar Lolić, Sandra Aranđelović, Milan Nikolić, Rada Baošić. "In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge" in Journal of Inorganic Biochemistry, Elsevier BV (2023). https://doi.org/10.1016/j.jinorgbio.2023.112224
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Synthesis, characterization, biomolecular interactions, molecular docking, and in vitro and in vivo anticancer activities of novel ruthenium(III) Schiff base complexes
Milica Međedović, Aleksandar Mijatović, Rada Baošić, Dejan Lazić, Žiko Milanović, Zoran Marković, Jelena Milovanović, Dragana Arsenijević, Bojana Stojanović, Miloš Arsenijević, Marija Milovanović, Biljana Petrović, Ana Rilak Simović (2023)In order to discover new anticancer drugs, novel ruthenium(III) complexes [Ru(L)Cl(H2O)], where L is tetradentate Schiff base bis(acetylacetone)ethylendiimine (acacen, 1), bis(benzoylacetone)ethylendiimine (bzacen, 2), (acetylacetone)(benzoylaceton)ethylendiimine (acacbzacen, 3), bis(acetylacetone)propylendiimine (acacpn, 4), bis(benzoylacetone)propylendiimine (bzacpn, 5) or (acetylacetone)(benzoylaceton)propylendiimine (acacbzacpn, 6), were synthesized. The complexes 1 – 6 were characterized by elemental analysis, molar conductometry, and by various spectroscopic techniques, such as UV–Vis, IR, EPR, and ESI-MS. Based on in vitro DNA/BSA experiments, complexes 2 (bzacen) and 5 (bzacpn) with two aromatic rings showed ...Milica Međedović, Aleksandar Mijatović, Rada Baošić, Dejan Lazić, Žiko Milanović, Zoran Marković, Jelena Milovanović, Dragana Arsenijević, Bojana Stojanović, Miloš Arsenijević, Marija Milovanović, Biljana Petrović, Ana Rilak Simović. "Synthesis, characterization, biomolecular interactions, molecular docking, and in vitro and in vivo anticancer activities of novel ruthenium(III) Schiff base complexes" in Journal of Inorganic Biochemistry, Elsevier BV (2023). https://doi.org/10.1016/j.jinorgbio.2023.112363
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Crystallographic, spectroscopic, thermal and computational studies of polymeric cobalt(II)–mellitate complex with 2,2′-bipyridine
Orange single crystals of new polymeric cobalt(II) complex {[Co(bipy)(H2O)4]2[Co(μ-mell)(H2O)2]·10H2O}n, 1, were synthesized by slow evaporation method at room temperature (bipy = 2,2′-bipyridine, mell = hexaanion of mellitic acid) and its crystal structure was determined by single-crystal X-ray diffraction. The complex 1 was characterized based on elemental analysis, FTIR spectroscopy and thermal (TG/DTA) analysis followed by computational analysis of noncovalent interactions and quantum chemical calculations of interaction energies. In 1, two crystallographically different Co(II) atoms adopt a deformed octahedral geometry, ...Lidija Radovanović, Dušan P. Malenov, Marko V. Rodić, Aleksandar Kremenović, Jelena Rogan. "Crystallographic, spectroscopic, thermal and computational studies of polymeric cobalt(II)–mellitate complex with 2,2′-bipyridine" in Journal of Molecular Structure, Elsevier BV (2021). https://doi.org/10.1016/j.molstruc.2021.132202
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NMR kinetic studies of the interactions between [Ru(terpy)(bipy)(H2O)]2+ and some sulfur-donor ligands
Aleksandar Mijatović, Biljana Šmit, Ana Rilak, Biljana Petrović, Dragan Čanović, Živadin D. Bugarčić (2012)Reakcije supstitucije monofunkcionalnog [Ru(terpi)(bipi)(H2O)]2+ kompleksa, gde je terpi = 2,2′:6′,2″-terpiridin i bipi = 2,2′-bipiridin, sa biološki relevantnim sumporo donorskim ligandima, kao što su tiourea, l-metionin, l-cistein i glutation, proučavane su u vodenim rastvorima tehnikom 1H i 13C NMR spektroskopije. Sve reakcije su proučavajne na pH 7,4, u prisustvu fosfatnog pufera, da bi se oponašalo fiziološko okruženje tokom procesa supstitucije. Reakcije se izvode na 295 K pod uslovima reakcija drugog reda. Uočeni rezultati pokazuju da brzina supstitucije snažno zavisi ...Aleksandar Mijatović, Biljana Šmit, Ana Rilak, Biljana Petrović, Dragan Čanović, Živadin D. Bugarčić. "NMR kinetic studies of the interactions between [Ru(terpy)(bipy)(H2O)]2+ and some sulfur-donor ligands" in Inorganica Chimica Acta, Elsevier BV (2012). https://doi.org/10.1016/j.ica.2012.09.016
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Substitution reactions of some novel sterically hindered monofunctional Pd(II) complexes
Reakcije supstitucije monofunkcionalnih kompleksa [(TLtBu)PdCl]+ i [Pd(tpdm)Cl]+, gde je TLtBu = 2,6-bis[(1,3-di-terc-butilimidazolin-2-imino)metil]piridin i tpdm = terpiridindimetan, sa nukleofilima kao što su: tiourea, I−, Br−, NO2−, piridin i dimetil-sulfoksid (DMSO) proučavani su u 0,1 M vodenom rastvoru NaClO4 u prisustvu 10 mM NaCl. Reakcije su sprovedene na tri različite temperature (288, 298 i 308 K) korišćenjem tehnike zaustavljenog protoka na različitim temperaturama. Reakcije supstitucije su praćene pod uslovima pseudo prvog reda sa velikim viškom nukleofila. Dobijeni rezultati pokazuju da kompleks ...... W.B. Tolman, M. Tamm, Dalton Trans. (2008) 887. [27] T.G. Appleton, J.R. Hall, S.F. Ralph, C.S.M. Thompson, Inorg. Chem. 23 (1984) 3521. [28] Inorganic Reaction Mechanisms, in: M.L. Tobe and J. Burgess (Ed.), Addison Wesley Longman Inc., Essex, 1999, p. 74. [29] P. Illner, S. Kern, S. Begel, R. van ...Aleksandar Mijatović, Jovana Bogojeski, Biljana Petrović, Živadin D. Bugarčić. "Substitution reactions of some novel sterically hindered monofunctional Pd(II) complexes" in Inorganica Chimica Acta, Elsevier BV (2011). https://doi.org/10.1016/j.ica.2011.11.031
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Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?
Lena Platanić Arizanović, Nikola Gligorijević, Ilija Cvijetić, Aleksandar Mijatović, Maja Krstić Ristivojević, Simeon Minić, Aleksandra Nikolić Kokić, Čedo Miljević, Milan Nikolić (2023)Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes are a suitable model system for testing the pleiotropic effects of lipophilic drugs. Our study investi gated the interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and human hemoglobin under simulated physiological conditions. Analysis of protein fluorescence quenching at different temperatures and data obtained from the van’t Hoff diagram and molecular docking indicate that the interactions are static and that the tetrameric human hemoglobin has one binding site for ...Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, CatalysisLena Platanić Arizanović, Nikola Gligorijević, Ilija Cvijetić, Aleksandar Mijatović, Maja Krstić Ristivojević, Simeon Minić, Aleksandra Nikolić Kokić, Čedo Miljević, Milan Nikolić. "Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?" in International Journal of Molecular Sciences, MDPI AG (2023). https://doi.org/10.3390/ijms24108921
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Novel cerium and praseodymium doped phosphate tungsten bronzes: Synthesis, characterization, the behavior in the Briggs-Rauscher reaction and photoluminescence properties
Tijana Maksimović, Pavle Tančić, Jelena Maksimović, Dimitrije Mara, Marija Ilić, Rik Van Deun, Ljubinka Joksović, Maja Pagnacco (2023)Due to the interesting and potentially useful properties, phosphate tungsten bronzes are constantly being studied and attract a lot of attention. In the present work, two different metallic elements, belonging to the group of rare-earth metals, cerium and praseodymium, were used as a dopants for phosphate tungsten bronzes. Novel cerium and praseodymium doped phosphate tungsten bronzes were successfully synthesized and further characterized by thermal analyses, Fourier-transform infrared spectroscopy, X-ray powder diffraction, scanning electron microscopy with energy-dispersive X-ray spectrometer and ...Electrical and Electronic Engineering, Atomic and Molecular Physics and Optics, Electronic, Optical and Magnetic Materials, Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, SpectroscopyTijana Maksimović, Pavle Tančić, Jelena Maksimović, Dimitrije Mara, Marija Ilić, Rik Van Deun, Ljubinka Joksović, Maja Pagnacco. "Novel cerium and praseodymium doped phosphate tungsten bronzes: Synthesis, characterization, the behavior in the Briggs-Rauscher reaction and photoluminescence properties" in Optical Materials, Elsevier BV (2023). https://doi.org/10.1016/j.optmat.2023.114125
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Crystallography and Surface Oxidation of Stoichiometric Arsenopyrite from Šumadija-Kopaonik Pb-Zn/Polymetallic Ore District (Serbia)
Ivana Jelić, Aleksandar Pačevski, Aleksandar Kremenović, Aleš Šoster, Andreja Šestan, Janez Zavašnik (2023)The behaviour and stability of arsenopyrite largely depend on its crystal structure, but studies of crystallography and structural defects are scarce. For clarification, we investigated a series of natural arsenopyrite crystals from the polymetallic Pb-Zn-(±Cu,Ag,Au,Sb,Bi,W) Šumadija-Kopaonik ore district (Serbia) by powder X-ray diffraction (XRPD). Among them, samples from the Drenjak gold mineralisation showed near-stoichiometric FeAsS composition and were further investigated by conventional and high-resolution transmission electron microscopy methods. TEM revealed abundant planar crystallographic defects and epitaxial surface oxide layer ...Inorganic Chemistry, Condensed Matter Physics,General Materials Science, General Chemical Engineering, crystal structure, FeAsS, oxidation, TEM, XRPD... potential ecological concerns due to As release through sulphide weathering and oxidation. The existing, research on arsenopyrite mainly focuses on the chemistry, processing, and weathering of the Au-containing arsenic sulphide minerals [4]. The ability to incorporate Au in FeAsS structure coincides with ...
... arsenopyrite crystal structure. Conflicts of Interest: The authors declare no conflict of interest. References 1. · Vaughan, D.J.;Craig, J. Mineral Chemistry of Metal Sulfides; Cambridgjee University Press: Cambridge, UK, 1978. 2. · Merkulova, M.; Mathon, O.; Glatzel, P.; Rovezzi, M.; Batanova, V.; Marion ...Ivana Jelić, Aleksandar Pačevski, Aleksandar Kremenović, Aleš Šoster, Andreja Šestan, Janez Zavašnik. "Crystallography and Surface Oxidation of Stoichiometric Arsenopyrite from Šumadija-Kopaonik Pb-Zn/Polymetallic Ore District (Serbia)" in Crystals, MDPI AG (2023). https://doi.org/10.3390/cryst13020278
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Ionothermal synthesis and crystal structure of a new organic–inorganic hybrid compound:catena-poly[bis(1-ethyl-3-methylimidazolium) [hepta-μ-bromido-pentacuprate(I)]]
A new organic-inorganic hybrid compound, catena-poly[bis(1-ethyl-3-methylimidazolium) [μ5-bromido-tri-μ3-bromido-tri-μ2-bromido-pentacuprate(I)]], {(C6H11N2)2[Cu5Br7]}n, has been obtained under ionothermal conditions from a reaction mixture containing Ba(OH)2•8H O, Cu(OH)2•2H2O, As2O5 , 1-ethyl-3-methylimidazolium bromide and distilled water. The crystal structure consists of complex [Cu5Br7]2- anions arranged in sinusoidal {[Cu5Br7]2-}n chains running along the a axis, which are surrounded by 1-ethyl-3-methylimidazolium cations. Three of the five unique Br atoms and one of the three Cu(I) atoms occupy special positions with half-occupancy (a mirror plane perpendicular to the b ...кристална структура, органско-неорганско хибридно једињење, бакар(I) бромид, јонотермна синтеза, 1-етил-3-метилимидазолијум бромид, Cu•••Cu интеракцијеLjiljana Karanović, Dejan Poleti, Tamara Đorđević. "Ionothermal synthesis and crystal structure of a new organic–inorganic hybrid compound:catena-poly[bis(1-ethyl-3-methylimidazolium) [hepta-μ-bromido-pentacuprate(I)]]" in Acta Crystallographica Section C Structural Chemistry, International Union of Crystallography (IUCr) (2014). https://doi.org/10.1107/S205322961402172X
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An overview of the radiation properties of spring water in the rural areas of Central Serbia
Biljana Vučković, Smiljana Marković, Snežana Stević, Sanja Mrazovac Kurilić, Ljiljana Nikolić-Bujanović, Nataša Todorović, Jovana Nikolov, Dragan Radovanović, Danica Srećković Batoćanin, Anja Jokić (2021)Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Pollution,Soil Science,Waste Management and Disposal,Water Science and Technology,Environmental Chemistry,Analytical ChemistryBiljana Vučković, Smiljana Marković, Snežana Stević, Sanja Mrazovac Kurilić, Ljiljana Nikolić-Bujanović, Nataša Todorović, Jovana Nikolov, Dragan Radovanović, Danica Srećković Batoćanin, Anja Jokić. "An overview of the radiation properties of spring water in the rural areas of Central Serbia" in International Journal of Environmental Analytical Chemistry, Informa UK Limited (2021). https://doi.org/10.1080/03067319.2021.1890060
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Geochemical properties of the sediments of the Great War Island, Belgrade (Serbia)
Zarić Nenad, Šajnovič Aleksandra, Jovančićević Branislav, Baltić Nevena, Ranđelović Dragana, Kašanin-Grubin Milica (2017)Zarić Nenad, Šajnovič Aleksandra, Jovančićević Branislav, Baltić Nevena, Ranđelović Dragana, Kašanin-Grubin Milica. "Geochemical properties of the sediments of the Great War Island, Belgrade (Serbia)" in Book of Abstracts of the18th European Meeting on Environmental Chemistry - EMEC18: Chemistry Towards an Infinite Environment, :Association of Chemistry and the Environment (2017): 241-242
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Organoselenium functionalized oxacycles as ligands in some trans-palladium(II) complexes: biological evaluation and interaction with small biomolecules
I. Raković, D. Ćoćić, O. Milošević-Djordjević, I. Radojević, M. N. Živanović, K. Virijević, J. Pavić, J. Tubić Vukajlović, A. Marković, J. S. Marjanović, M. D. Kostić, P. Čanović, A. Mijatović, V. M. Divac (2023)In this article, the biological evaluation and interaction of three selenium-based trans-palladium complexes (Pd–Se1, Pd–Se2, and Pd–Se3) with small biomolecules were performed. The UV–Vis kinetic studies on substitution reaction of synthesized complexes with selected biologically important N- and S-bonding ligands (LCys, GSH, L-Met, 50-GMP, L-His) has emphasized greater affinity of sulphur-based ligands for complex-binding, while the general order of the reactivity was L-Cys > GSH > L-Met > 50-GMP > L-His. The complex reactivity toward small biomolecules is influenced ...I. Raković, D. Ćoćić, O. Milošević-Djordjević, I. Radojević, M. N. Živanović, K. Virijević, J. Pavić, J. Tubić Vukajlović, A. Marković, J. S. Marjanović, M. D. Kostić, P. Čanović, A. Mijatović, V. M. Divac. "Organoselenium functionalized oxacycles as ligands in some trans-palladium(II) complexes: biological evaluation and interaction with small biomolecules" in Journal of Coordination Chemistry, Informa UK Limited (2023). https://doi.org/10.1080/00958972.2023.2256934
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Mean-based iterative methods for finding multiple roots in nonlinear chemistry problems
Dejan Ćebić, Nebojša M. Ralević (2021)Dejan Ćebić, Nebojša M. Ralević. "Mean-based iterative methods for finding multiple roots in nonlinear chemistry problems" in Journal of Mathematical Chemistry, Springer Science and Business Media LLC (2021). https://doi.org/10.1007/s10910-021-01253-3
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Investigation of Co0.9Ho0.1MoO4 Nanopowders Obtained by Glycine Nitrate Procedure
Milena Rosić, Maja Milošević, Maria Čebela, Vladimir Dodevski, Vesna Lojpur, Radomir Ljupković, Aleksandra Zarubica (2023)... of CoooHooMoOu, as well as a possible new approach in inorganic synthesis methodology. During photocatalytic testing, the studied nanoparticle powder indicated a potentially i promising solution in photocatalytic processes toward green chemistry and sustainable development. Keywords: X-ray diffraction ...
... N. Macedonia Members: Prof. Dr. Trajče Stafilov, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University in Skopje, R. N. Macedonia Prof. Dr. Viktor Stefov, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius ...
... in Skopje, R. N. Macedonia Ivona Sofronievska, MSc, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University in Skopje, R. Macedonia Marinela Cvetanoska, MSc, Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius ...Milena Rosić, Maja Milošević, Maria Čebela, Vladimir Dodevski, Vesna Lojpur, Radomir Ljupković, Aleksandra Zarubica. "Investigation of Co0.9Ho0.1MoO4 Nanopowders Obtained by Glycine Nitrate Procedure" in Book of Abstracts / 26th Congress of SCTM with international participation, 20-23 September 2023, Ohrid, R. Macedonia, Skopje : Society of Chemists and Technologists of Macedonia (2023)
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Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases
The infuences of the application of mono- and two-component organic modifers on lipophilicity determination of 12 tetradentate Schif bases by reversed-phase thin layer chromatography were investigated. The main goal is to estimate types of interaction between observed compounds and components of the applied chromatographic systems and establish some behaviour pattern in order to easier choose a combination of organic modifers which will simulate interaction in biological systems based on the facts that the same basic intermolecular interactions are responsible ...Materials Chemistry, Industrial and Manufacturing Engineering, General Chemical Engineering, Biochemistry,General ChemistryNikola Stevanović, Aleksandar Mijatović, Aleksandar Lolić, Mario Zlatović, Rada Baošić. "Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases" in Chemical Papers, Springer Science and Business Media LLC (2021). https://doi.org/10.1007/s11696-021-01884-5
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Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases
The infuences of the application of mono- and two-component organic modifers on lipophilicity determination of 12 tet radentate Schif bases by reversed-phase thin layer chromatography were investigated. The main goal is to estimate types of interaction between observed compounds and components of the applied chromatographic systems and establish some behaviour pattern in order to easier choose a combination of organic modifers which will simulate interaction in biological systems based on the facts that the same basic intermolecular interactions are ...Materials Chemistry, Industrial and Manufacturing Engineering, General Chemical Engineering, Biochemistry, General ChemistryNikola Stevanović, Aleksandar Mijatović, Aleksandar Lolić, Mario Zlatović, Rada Baošić. "Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases" in Chemical Papers, Springer Science and Business Media LLC (2021). https://doi.org/10.1007/s11696-021-01884-5
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Physical-chemistry characterization of the modified limestone
Mihajlović Slavica, Vučinić Dušica, Sekulić Živko, Jovanović Vladimir, Radulović Dragan. "Physical-chemistry characterization of the modified limestone" in Proceedings of 5th BALKAN MINING CONGRESS, Ohrid, Macedonia: (2013): 386-391
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Crystal chemistry of some KF flux grown REE-silicates
Dabić Predrag, Vulić Predrag, Kahlenberg Volker, Kremenović Aleksandar. "Crystal chemistry of some KF flux grown REE-silicates" in XXII Conference of the Serbian Crystallographic Society, Kragujevac, Srbija:Srpsko kristalografsko društvo (2015): 24-25