Collected Item: “In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge”
Врста публикације
Рад у часопису
Верзија рада
објављена верзија
Језик рада
енглески
Аутор/и (Милан Марковић, Никола Николић)
Aleksandar Mijatović, Nevenka Gligorijević, Dušan Ćoćić, Snežana Spasić, Aleksandar Lolić, Sandra Aranđelović, Milan Nikolić, Rada Baošić
Наслов рада (Наслов - поднаслов)
In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge
Наслов часописа
Journal of Inorganic Biochemistry
Издавач (Београд : Просвета)
Elsevier BV
Година издавања
2023
Сажетак на енглеском језику
The biological activity of six structurally similar tetradentate Schiff base copper(II) complexes, namely [Cu(ethylenediamine-bis-acetylacetonate)] (CuAA) and five derivatives where two methyl groups are replaced by phenyl, (CuPP), CF3 (CuTT) or by mixed groups CH3/CF3 (CuAT), Ph/CF3 (CuPT), and Ph/CH3 (CuAP) has been investigated. The set of antioxidant assays was performed, and the results were expressed as IC50 and EC50 values. The series of complexes showed interesting bioactivity and were investigated for the determination of antioxidant, antifungal, antimicrobial, and cytotoxic activity. A significant antioxidant behavior was exhibited by complex CuAA, greater than Trolox in the Oxygen Radical Absorbance Capacity (ORAC) assay. Antibacterial assay over Gram-positive and Gram-negative pathogenic bacterial strains and some fungal pathogens were studied. Antiproliferative activity of complexes in two human tumor cell lines, breast adenocarcinoma MCF-7, colon adenocarcinoma LS-174, and normal fibroblast cells-MRC-5, examined the effect on cell cycle progression. The significant cytotoxic potential, comparable to cisplatin cytotoxicity, was determined in human breast cancer cell line-MCF-7 with IC50 values being 17.53–31.40 μM and human colon cancer cell line-LS-174 with IC50
values being 15.22–23.92 μM. All tested compounds showed nearly twice more selectivity toward cancer cell lines than normal cells. The interactions of complexes with human serum albumin (HSA), the most prominent protein in plasma, were investigated using spectroscopic fluorescence techniques. The complexes bind to human serum albumin at multiple sites (n = 0.2–1.9), displaying a moderate binding constant Ka = 4.1–12.4 × 104 M− 1. The molecular docking experiment effectively showed complex binding to HSA and DNA molecular fragments.
values being 15.22–23.92 μM. All tested compounds showed nearly twice more selectivity toward cancer cell lines than normal cells. The interactions of complexes with human serum albumin (HSA), the most prominent protein in plasma, were investigated using spectroscopic fluorescence techniques. The complexes bind to human serum albumin at multiple sites (n = 0.2–1.9), displaying a moderate binding constant Ka = 4.1–12.4 × 104 M− 1. The molecular docking experiment effectively showed complex binding to HSA and DNA molecular fragments.
Волумен/том или годиште часописа
244
Број часописа
-
DOI број
10.1016/j.jinorgbio.2023.112224
ISSN број часописа
0162-0134
Кључне речи на српском (одвојене знаком ", ")
Inorganic Chemistry,Biochemistry
Кључне речи на енглеском (одвојене знаком ", ")
Inorganic Chemistry,Biochemistry
Линк
https://api.elsevier.com/content/article/PII:S016201342300106X?httpAccept=text/xml
Шира категорија рада према правилнику МПНТ
M20
Ужа категорија рада према правилнику МПНТ
М21
Степен доступности
Затворени приступ
Лиценца
Creative Commons – Attribution-No Derivative Works 4.0 International
Формат дигиталног објекта
.pdf